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首页> 外文OA文献 >Overexpression of a Maize Sulfite Oxidase Gene in Tobacco Enhances Tolerance to Sulfite Stress via Sulfite Oxidation and CAT-Mediated H2O2 Scavenging
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Overexpression of a Maize Sulfite Oxidase Gene in Tobacco Enhances Tolerance to Sulfite Stress via Sulfite Oxidation and CAT-Mediated H2O2 Scavenging

机译：烟草中玉米亚硫酸盐氧化酶基因的过表达通过亚硫酸盐氧化和CAT介导的H2O2清除增强对亚硫酸盐胁迫的耐受性

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Sulfite oxidase (SO) plays an important role in sulfite metabolism. To date, the molecular mechanisms of sulfite metabolism in plants are largely unknown. Previously, a full-length cDNA of the putative sulfite oxidase gene from maize (ZmSO) was cloned, and its response to SO2/sulfite stress at the transcriptional level was characterized. In this study, the recombinant ZmSO protein was purified from E.coli. It exhibited sulfite-dependent activity and had strong affinity for the substrate sulfite. Over-expression (OE) of ZmSO in tobacco plants enhanced their tolerance to sulfite stress. The plants showed much less damage, less sulfite accumulation, but greater amounts of sulfate. This suggests that tolerance of transgenic plants to sulfite was enhanced by increasing SO expression levels. Interestingly, H2O2 accumulation levels by histochemical detection and quantitative determination in the OE plants were much less than those in the wild-type upon sulfite stress. Furthermore, reductions of catalase levels detected in the OE lines were considerably less than in the wild-type plants. This indicates that SO may play an important role in protecting CAT from inhibition by excess sulfite. Collectively, these data demonstrate that transgenic tobacco plants over-expressing ZmSO enhance tolerance to excess sulfite through sulfite oxidation and catalase-mediated hydrogen peroxide scavenging. This is the first SO gene from monocots to be functionally characterized.
机译：亚硫酸盐氧化酶（SO）在亚硫酸盐代谢中起重要作用。迄今为止，还不清楚植物中亚硫酸盐代谢的分子机制。先前，克隆了玉米推定的亚硫酸盐氧化酶基因的全长cDNA（ZmSO），并在转录水平上表征了其对SO2 /亚硫酸盐胁迫的响应。在这项研究中，重组ZmSO蛋白是从大肠杆菌中纯化的。它具有亚硫酸盐依赖性的活性，并且对底物亚硫酸盐具有很强的亲和力。 ZmSO在烟草植物中的过表达（OE）增强了其对亚硫酸盐胁迫的耐受性。这些植物显示出更少的损害，更少的亚硫酸盐积累，但更多的硫酸盐含量。这表明通过增加SO表达水平来提高转基因植物对亚硫酸盐的耐受性。有趣的是，在亚硫酸盐胁迫下，通过组织化学检测和定量测定，OE植物中H2O2的积累水平远低于野生型中的水平。此外，在OE系中检测到的过氧化氢酶水平的降低明显小于野生型植物。这表明SO可能在保护CAT免受过量亚硫酸盐的抑制作用中起重要作用。总而言之，这些数据表明，过表达ZmSO的转基因烟草植物通过亚硫酸盐氧化和过氧化氢酶介导的过氧化氢清除增强了对过量亚硫酸盐的耐受性。这是来自单子叶植物的第一个SO基因，具有功能特征。

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